Introduction:

RNA-Seq, short for RNA sequencing, is a powerful technique that uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA molecules in a biological sample. It provides a snapshot of gene expression in the sample, which is also known as the transcriptome. Specifically, RNA-Seq allows researchers to explore alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutations, and changes in gene expression over time. It can analyze various types of RNA, including mRNA, small RNA (such as miRNA), and ribosomal profiling.